The Human (HIF1AN) Hypoxia-inducible Factor 1-alpha Inhibitor ELISA Kit measures Hypoxia-Inducible Factor 1-Alpha Inhibitor in samples. The plate has been pre-coated with Human HIF1AN antibody. HIF1AN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HIF1AN Antibody is added and binds to HIF1AN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF1AN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HIF1AN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundHydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. Source: UniProt Consortium (2025)
