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Human HIST1H4A (Histone H4) ELISA Kit

Human HIST1H4A (Histone H4) ELISA Kit

The Human (HIST1H4A) Histone H4 ELISA Kit measures Histone H4 in samples. The plate has been pre-coated with Human H4C1 antibody. H4C1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human H4C1 Antibody is added and binds to H4C1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated H4C1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human H4C1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5420Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 10.33 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Epigenetics and Nuclear Signaling
Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Histone H4
Regulatory Status For Research Use Only
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