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Human HLA-C (Leukocyte Antigen C) ELISA Kit
Human HLA-C (Leukocyte Antigen C) ELISA Kit
The Human (HLA-C) Leukocyte Antigen C ELISA Kit measures Leukocyte Antigen C in samples. The plate has been pre-coated with Human HLA-C antibody. HLA-C present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HLA-C Antibody is added and binds to HLA-C in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HLA-C antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HLA-C. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0436Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.2 ng/mL
Detection Range
0.5-150 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immune molecule
Background
Antigen-presenting major histocompatibility complex class I (MHCI) molecule with an important role in reproduction and antiviral immunity (PubMed: 20972337, PubMed: 24091323, PubMed: 20439706, PubMed: 11172028, PubMed: 20104487, PubMed: 28649982, PubMed: 29312307). In complex with B2M/beta 2 microglobulin displays a restricted repertoire of self and viral Peptides and acts as a dominant ligand for inhibitory and activating killer immunoglobulin receptors (KIRs) expressed on NK cells (PubMed: 16141329). In an allogeneic setting, such as during pregnancy, mediates interaction of extravillous trophoblasts with KIR on uterine NK cells and regulate trophoblast invasion necessary for placentation and overall fetal growth (PubMed: 20972337, PubMed: 24091323). During viral infection, may present viral Peptides with low affinity for KIRs, impeding KIR-mediated inhibition through Peptide antagonism and favoring lysis of infected cells (PubMed: 20439706). Presents a restricted repertoire of viral Peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-C-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected cells, particularly in chronic viral infection settings such as HIV-1 or CMV infection (PubMed: 11172028, PubMed: 20104487, PubMed: 28649982). Both the Peptide and the MHC molecule are recognized by TCR, the Peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (By similarity). Typically presents intracellular Peptide antigens of 9 amino acids that arise from cytosolic proteolysis via proteasome. Can bind different Peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9. Preferentially displays Peptides having a restricted repertoire of hydrophobic or aromatic amino acids (Phe, Ile, Leu, Met, Val and Tyr) at the C-terminal anchor (PubMed: 8265661, PubMed: 25311805). ALLELE C*01:02: The Peptide-bound form interacts with KIR2DL2 and KIR2DL3 inhibitory receptors on NK cells. The low affinity Peptides compete with the high affinity Peptides impeding KIR-mediated inhibition and favoring lysis of infected cells (PubMed: 20439706). Presents to CD8-positive T cells a CMV epitope derived from UL83/pp65 (RCPEMISVL), an immediate-early antigen necessary for initiating viral replication (PubMed: 12947002). ALLELE C*04:01: Presents a conserved HIV-1 epitope derived from env (SFNCGGEFF) to memory CD8-positive T cells, eliciting very strong IFNG responses (PubMed: 20104487). Presents CMV epitope derived from UL83/pp65 (QYDPVAALF) to CD8-positive T cells, triggering T cell cytotoxic response (PubMed: 12947002). ALLELE C*05:01: Presents HIV-1 epitope derived from rev (SAEPVPLQL) to CD8-positive T cells, triggering T cell cytotoxic response. ALLELE C*06:02: In trophoblasts, interacts with KIR2DS2 on uterine NK cells and triggers NK cell activation, including secretion of cytokines such as GMCSF that enhances trophoblast migration. ALLELE C*07:02: Plays an important role in the control of chronic CMV infection. Presents immunodominant CMV epitopes derived from IE1 (LSEFCRVL and CRVLCCYVL) and UL28 (FRCPRRFCF), both antigens synthesized during immediate-early period of viral replication. Elicits a strong anti-viral CD8-positive T cell immune response that increases markedly with age. ALLELE C*08:01: Presents viral epitopes derived from CMV UL83 (VVCAHELVC) and IAV M1 (GILGFVFTL), triggering CD8-positive T cell cytotoxic response. ALLELE C*12:02: Presents CMV epitope derived from UL83 (VAFTSHEHF) to CD8-positive T cells. ALLELE C*15:02: Presents CMV epitope derived from UL83 CC (VVCAHELVC) to CD8-positive T cells, triggering T cell cytotoxic response. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Leukocyte Antigen C
Regulatory Status
For Research Use Only
