The Human (HNRNP A1) Heterogeneous Nuclear Ribonucleoprotein A1 ELISA Kit measures Heterogeneous Nuclear Ribonucleoprotein A1 in samples. The plate has been pre-coated with Human HNRNPA1 antibody. HNRNPA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HNRNPA1 Antibody is added and binds to HNRNPA1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HNRNPA1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HNRNPA1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection (PubMed: 17371836). May bind to specific miRNA hairpins (PubMed: 28431233). Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1 (PubMed: 31$498.00|$360.00791). (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis. Source: UniProt Consortium (2025)
