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Human HPRT1 (Hypoxanthine-Guanine Phosphoribosyltransferase) ELISA Kit

Human HPRT1 (Hypoxanthine-Guanine Phosphoribosyltransferase) ELISA Kit

The Human (HPRT1) Hypoxanthine-Guanine Phosphoribosyltransferase ELISA Kit measures Hypoxanthine-Guanine Phosphoribosyltransferase in samples. The plate has been pre-coated with Human HPRT1 antibody. HPRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HPRT1 Antibody is added and binds to HPRT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HPRT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HPRT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E3638Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.025 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Epigenetics and Nuclear Signaling
Background Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Hypoxanthine-Guanine Phosphoribosyltransferase
Regulatory Status For Research Use Only
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