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Human HPT/HP (Haptoglobin/Zonulin) ELISA Kit
Human HPT/HP (Haptoglobin/Zonulin) ELISA Kit
The Human (HPT/HP) Haptoglobin/Zonulin ELISA Kit measures Haptoglobin/Zonulin in samples. The plate has been pre-coated with Human HP antibody. HP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HP Antibody is added and binds to HP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1117Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
1.09 ug/mL
Detection Range
2-600 ug/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Haptoglobin/Zonulin
Regulatory Status
For Research Use Only
