1
/
of
1
Human HTR1A (5-Hydroxytryptamine Receptor 1A) ELISA Kit
Human HTR1A (5-Hydroxytryptamine Receptor 1A) ELISA Kit
The Human (HTR1A) 5-Hydroxytryptamine Receptor 1A ELISA Kit measures 5-Hydroxytryptamine Receptor 1A in samples. The plate has been pre-coated with Human HTR1A antibody. HTR1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HTR1A Antibody is added and binds to HTR1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HTR1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HTR1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4882Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
0.026 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
5-Hydroxytryptamine Receptor 1A
Regulatory Status
For Research Use Only
