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Human IDE (Insulin-Degrading Enzyme) ELISA Kit
Human IDE (Insulin-Degrading Enzyme) ELISA Kit
The Human (IDE) Insulin-Degrading Enzyme ELISA Kit measures Insulin-Degrading Enzyme in samples. The plate has been pre-coated with Human IDE antibody. IDE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IDE Antibody is added and binds to IDE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IDE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IDE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5471Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
8.04 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience, Diabetes Research, Enzyme & Kinase, Endocrinology, Metabolism
Background
Plays a role in the cellular breakdown of insulin, APP Peptides, IAPP Peptides, natriuretic Peptides, glucagon, bradykinin, kallidin, and other Peptides, and thereby plays a role in intercellular Peptide signaling (PubMed: 2293021, PubMed: 10684867, PubMed: 26968463, PubMed: 17051221, PubMed: 17613531, PubMed: 18986166, PubMed: 19321446, PubMed: 23922390, PubMed: 24847884, PubMed: 26394692, PubMed: 29596046, PubMed: 21098034). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed: 23922390, PubMed: 26394692, PubMed: 29596046). Contributes to the regulation of Peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic Peptides that are secreted by neurons and microglia (PubMed: 9830016, PubMed: 26394692) (Probable). Degrades the natriuretic Peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed: 21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed: 21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic Peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Insulin-Degrading Enzyme
Regulatory Status
For Research Use Only
