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Human IFI35 (Interferon-induced 35 kDa Protein) ELISA Kit
Human IFI35 (Interferon-induced 35 kDa Protein) ELISA Kit
The Human (IFI35) Interferon-induced 35 kDa Protein ELISA Kit measures Interferon-Induced 35 Kda Protein in samples. The plate has been pre-coated with Human IFI35 antibody. IFI35 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IFI35 Antibody is added and binds to IFI35 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFI35 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IFI35. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5498Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.28 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology
Background
Acts as a signaling pathway regulator involved in innate immune system response (PubMed: 26342464, PubMed: 29038465, PubMed: 29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed: 10779520, PubMed: 10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed: 26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed: 29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed: 29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed: 29038465). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Interferon-Induced 35 Kda Protein
Regulatory Status
For Research Use Only
