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Human IFN-G (Interferon Gamma) ELISA Kit

Human IFN-G (Interferon Gamma) ELISA Kit

The Human (IFN-G) Interferon Gamma ELISA Kit measures Interferon Gamma in samples. The plate has been pre-coated with Human IFNG antibody. IFNG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IFNG Antibody is added and binds to IFNG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IFNG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IFNG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0105Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.49 ng/mL
Detection Range 1-400 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, Stem Cells, Cancer, Cytokines
Background Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interferon Gamma
Regulatory Status For Research Use Only
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