Human IGFBP-3 (Insulin-like Growth Factors Binding Protein 3) ELISA Kit
Human IGFBP-3 (Insulin-like Growth Factors Binding Protein 3) ELISA Kit
The Human (IGFBP-3) Insulin-like Growth Factors Binding Protein 3 ELISA Kit measures Insulin-like Growth Factors Binding Protein 3 in samples. The plate has been pre-coated with Human IGFBP3 antibody. IGFBP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IGFBP3 Antibody is added and binds to IGFBP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IGFBP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IGFBP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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