This Interleukin 6 ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human IL-6. Samples (orStandards) are added to the micro ELISA plate wells and combined with the specificantibody. Then a biotinylated detection antibody specific for Human IL-6 and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate welland incubated. Free components are washed away. The substrate solution is added to eachwell. Only those wells that contain Human IL-6, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction isterminated by the addition of stop solution and the color turns yellow. The optical density(OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value isproportional to the concentration of Human IL-6. You can calculate the concentration ofHuman IL-6 in the samples by comparing the OD of the samples to the standard curve.
Research AreasCancer, Metabolism, Immunology, Neuroscience, Cytokines
BackgroundThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human IL-6. Samples (orStandards) are added to the micro ELISA plate wells and combined with the specificantibody. Then a biotinylated detection antibody specific for Human IL-6 and AvidinHorseradish Peroxidase (HRP) conjugate are added successively to each micro plate welland incubated. Free components are washed away. The substrate solution is added to eachwell. Only those wells that contain Human IL-6, biotinylated detection antibody andAvidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction isterminated by the addition of stop solution and the color turns yellow. The optical density(OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value isproportional to the concentration of Human IL-6. You can calculate the concentration ofHuman IL-6 in the samples by comparing the OD of the samples to the standard curve.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
Unopened kits should be stored at 2 to 8C until expiration. If opened, follow the instructions in the IFU. Generally speaking, if opened store the microplate, standard, detection antibody and HRP conjugate at -20C.
