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Human IL10RA (Interleukin-10 Receptor Subunit Alpha) ELISA Kit
Human IL10RA (Interleukin-10 Receptor Subunit Alpha) ELISA Kit
The Human (IL10RA) Interleukin-10 Receptor Subunit Alpha ELISA Kit measures Interleukin-10 Receptor Subunit Alpha in samples. The plate has been pre-coated with Human IL10RA antibody. IL10RA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL10RA Antibody is added and binds to IL10RA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL10RA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL10RA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6471Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.023 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, Cytokines, Inflammation
Background
Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed: 16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed: 12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed: 26962683). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Interleukin-10 Receptor Subunit Alpha
Regulatory Status
For Research Use Only
