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Human IL1RAP (Interleukin-1 Receptor Accessory Protein) ELISA Kit
Human IL1RAP (Interleukin-1 Receptor Accessory Protein) ELISA Kit
The Human (IL1RAP) Interleukin-1 Receptor Accessory Protein ELISA Kit measures Interleukin-1 Receptor Accessory Protein in samples. The plate has been pre-coated with Human IL1RAP antibody. IL1RAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL1RAP Antibody is added and binds to IL1RAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL1RAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL1RAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5504Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.033 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cytokines, Inflammation, Immunology
Background
Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable). [Isoform 2]: Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (PubMed: 12530978). Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity). [Isoform 4]: Unable to mediate canonical IL-1 signaling (PubMed: 19481478). Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Interleukin-1 Receptor Accessory Protein
Regulatory Status
For Research Use Only
