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Human JMJD1A (Lysine-specific Demethylase 3A) ELISA Kit

Human JMJD1A (Lysine-specific Demethylase 3A) ELISA Kit

The Human (JMJD1A) Lysine-specific Demethylase 3A ELISA Kit measures Lysine-specific demethylase 3A in samples. The plate has been pre-coated with Human JMJD1A antibody. JMJD1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human JMJD1A Antibody is added and binds to JMJD1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated JMJD1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human JMJD1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E6783Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 3.48 ng/L
Detection Range 7-1500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Neuroscience, Signal Transduction
Background Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Lysine-specific demethylase 3A
Regulatory Status For Research Use Only
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