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Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
Human MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
The Human (MMP-9) Matrix Metalloproteinase 9 ELISA Kit measures Matrix Metalloproteinase 9/Gelatinase B in samples. The plate has been pre-coated with Human MMP-9 antibody. MMP- 9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MMP-9 Antibody is added and binds to MMP-9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP-9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MMP-9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0936Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
15.12 ng/L
Detection Range
30-9000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer, Cell Biology
Background
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005).
Could play a role in bone osteoclastic resorption (By similarity).
Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005).
Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094).
Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034).
Degrades fibronectin but not laminin or Pz-peptide. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Matrix Metalloproteinase 9/Gelatinase B
Regulatory Status
For Research Use Only
