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Human NEU3 (Sialidase-3) ELISA Kit
Human NEU3 (Sialidase-3) ELISA Kit
The Human (NEU3) Sialidase-3 ELISA Kit measures Sialidase-3 in samples. The plate has been pre-coated with Human NEU3 antibody. NEU3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEU3 Antibody is added and binds to NEU3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEU3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NEU3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3140Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.017 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:11298736, PubMed:15847605, PubMed:10861246, PubMed:20511247, PubMed:28646141, PubMed:10405317, PubMed:12011038).
Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR (PubMed:17334392, PubMed:25922362).
Desialylates EGFR and activates downstream signaling in proliferating cells (PubMed:25922362).
Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes (PubMed:26251452). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Sialidase-3
Regulatory Status
For Research Use Only
