Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit
Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit
The Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit measures NF-KB p65 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NF-KB p65 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NF-KB p65 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NF-KB p65 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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