Human NPHN (Nephrin) antibody ELISA Kit
Human NPHN (Nephrin) antibody ELISA Kit
The Human NPHN (Nephrin) antibody ELISA Kit measures Anti-NPHS1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with Anti-NPHS1 bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-NPHS1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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