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Human NRP1 (Neuropilin-1) ELISA Kit
Human NRP1 (Neuropilin-1) ELISA Kit
The Human (NRP1) Neuropilin-1 ELISA Kit measures Neuropilin-1 in samples. The plate has been pre-coated with Human NRP1 antibody. NRP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NRP1 Antibody is added and binds to NRP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NRP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NRP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2101Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.054 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed: 9288753, PubMed: 9529250, PubMed: 10688880). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed: 19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed: 9288753, PubMed: 9529250, PubMed: 10688880, PubMed: 19805273). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed: 30623799). [Isoform 2]: Binds VEGF-165 and may inhibit its binding to cells (PubMed: 10748121, PubMed: 26503042). May induce apoptosis by sequestering VEGF-165 (PubMed: 10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Neuropilin-1
Regulatory Status
For Research Use Only
