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Human Nucleoside Diphosphate Phosphatase Entpd5 (ENTPD5) ELISA Kit
Human Nucleoside Diphosphate Phosphatase Entpd5 (ENTPD5) ELISA Kit
This ELISA kit is designed to detect Human Nucleoside Diphosphate Phosphatase Entpd5 (Human ENTPD5). The assay plate has been pre-coated with mouse anti-Human ENTPD5 monoclonal antibody. When the sample containing ENTPD5 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human ENTPD5 Antibody is added to the wells and binds to ENTPD5 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human ENTPD5 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE182
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
6.72 pg/mL
Detection Range
23.4-1500 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction
Background
Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Nucleoside diphosphate phosphatase ENTPD5
Regulatory Status
For Research Use Only
