Human PDGFRBETA (Platelet Derived Growth Factor Receptor Beta) ELISA Kit
Human PDGFRBETA (Platelet Derived Growth Factor Receptor Beta) ELISA Kit
The Human PDGFRBETA (Platelet Derived Growth Factor Receptor Beta) ELISA Kit measures PDGFRB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PDGFRβ antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with PDGFRβ bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PDGFRβ in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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