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Human PS-1 (Presenilin 1) ELISA Kit
Human PS-1 (Presenilin 1) ELISA Kit
The Human (PS-1) Presenilin 1 ELISA Kit measures Presenilin 1 in samples. The plate has been pre-coated with Human PSEN1 antibody. PSEN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PSEN1 Antibody is added and binds to PSEN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PSEN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PSEN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5908Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.027 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed: 15274632, PubMed: 10545183, PubMed: 10593990, PubMed: 10206644, PubMed: 10899933, PubMed: 10811883, PubMed: 12679784, PubMed: 12740439, PubMed: 25043039, PubMed: 26280335, PubMed: 30598546, PubMed: 30630874, PubMed: 28269784, PubMed: 20460383). Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed: 15274632, PubMed: 25043039, PubMed: 26280335, PubMed: 30598546, PubMed: 30630874). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed: 9738936, PubMed: 10593990, PubMed: 10899933, PubMed: 10811883). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed: 11953314). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed: 11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed: 9738936, PubMed: 11953314). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity). Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2 (PubMed: 17428795, PubMed: 28269784). The holoprotein functions as a calcium-leak Channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis (PubMed: 25394380, PubMed: 16959576). Involved in the regulation of neurite outgrowth (PubMed: 15004326, PubMed: 20460383). Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Presenilin 1
Regulatory Status
For Research Use Only
