The Human (PTPN22) Tyrosine Protein Phosphate Non-receptor Type 22 ELISA Kit measures Tyrosine Protein Phosphate Non-Receptor Type 22 in samples. The plate has been pre-coated with Human PTPN22 antibody. PTPN22 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTPN22 Antibody is added and binds to PTPN22 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTPN22 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PTPN22. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundActs as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed: 16461343, PubMed: 18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed: 21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed: 16461343). Dephosphorylates the immune system activator SKAP2 (PubMed: 21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed: 23871208). Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed: 23991106). Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide) (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteTyrosine Protein Phosphate Non-Receptor Type 22
