The Human (PTPRN2) Receptor-type Tyrosine-protein Phosphatase N2 ELISA Kit measures Receptor-type tyrosine-protein phosphatase N2 in samples. The plate has been pre-coated with Human PTPRN2 antibody. PTPRN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTPRN2 Antibody is added and binds to PTPRN2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTPRN2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PTPRN2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPlays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (By similarity).
Required to maintain normal levels of renin expression and renin release (By similarity).
May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity).
Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity).
Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments (PubMed:26620550). Source: UniProt Consortium (2025)
