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Human Ribonuclease Pancreatic (RNASE1) ELISA Kit
Human Ribonuclease Pancreatic (RNASE1) ELISA Kit
This ELISA kit is designed to detect Human Ribonuclease Pancreatic (Human RNASE1). The assay plate has been pre-coated with mouse anti-Human Ribonuclease A monoclonal antibody. When the sample containing Ribonuclease A is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Ribonuclease A Antibody is added to the wells and binds to Ribonuclease A in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Ribonuclease A present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE271
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
26.26 pg/mL
Detection Range
62.5-4000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Ribonuclease pancreatic
Regulatory Status
For Research Use Only
