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Human SAT1 (Diamine Acetyltransferase 1) ELISA Kit

Human SAT1 (Diamine Acetyltransferase 1) ELISA Kit

The Human (SAT1) Diamine Acetyltransferase 1 ELISA Kit measures Diamine Acetyltransferase 1 in samples. The plate has been pre-coated with Human SAT1 antibody. SAT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SAT1 Antibody is added and binds to SAT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SAT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SAT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5181Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.027 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Metabolism
Background Enzyme which catalyzes the acetylation of polyamines (PubMed: 15283699, PubMed: 16455797, PubMed: 17516632). Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine (PubMed: 17516632). This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines (PubMed: 16455797). Also involved in the regulation of polyamine transport out of cells (PubMed: 16455797). Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine (PubMed: 16455797, PubMed: 17516632). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Diamine Acetyltransferase 1
Regulatory Status For Research Use Only
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