1
/
of
1
Human SDF-1B (Stromal Cell Derived Factor 1Beta) ELISA Kit
Human SDF-1B (Stromal Cell Derived Factor 1Beta) ELISA Kit
The Human (SDF-1B) Stromal Cell Derived Factor 1Beta ELISA Kit measures Stromal Cell Derived Factor 1 beta in samples. The plate has been pre-coated with Human CXCL12 antibody. CXCL12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CXCL12 Antibody is added and binds to CXCL12 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CXCL12 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CXCL12. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0083Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
33.53 ng/L
Detection Range
70-15000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, Microbiology
Background
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed: 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Stromal Cell Derived Factor 1 beta
Regulatory Status
For Research Use Only
