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Human SEMA4A (Semaphorin 4A) ELISA Kit

Human SEMA4A (Semaphorin 4A) ELISA Kit

The Human (SEMA4A) Semaphorin 4A ELISA Kit measures Semaphorin 4A in samples. The plate has been pre-coated with Human SEMA4A antibody. SEMA4A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4A Antibody is added and binds to SEMA4A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2062Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.046 ng/mL
Detection Range 0.1-40 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular
Background Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling (By similarity). Regulates glutamatergic and GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner (By similarity). Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity (By similarity). Promotes phosphorylation of TIMD2 (By similarity). Inhibits angiogenesis (By similarity). Promotes axon growth cone collapse (By similarity). Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Semaphorin 4A
Regulatory Status For Research Use Only
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