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Human SEMA4F (Semaphorin 4F) ELISA Kit

Human SEMA4F (Semaphorin 4F) ELISA Kit

The Human (SEMA4F) Semaphorin 4F ELISA Kit measures Semaphorin 4F in samples. The plate has been pre-coated with Human SEMA4F antibody. SEMA4F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4F Antibody is added and binds to SEMA4F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA4F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1565Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 3.06 ng/L
Detection Range 5-1000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Probable cell surface receptor that regulates oligodendroglial precursor cell migration (By similarity). Might also regulate differentiation of oligodendroglial precursor cells (By similarity). Has growth cone collapse activity against retinal ganglion-cell axons (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Semaphorin 4F
Regulatory Status For Research Use Only
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