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Human SEMA5A (Semaphorin 5A) ELISA Kit
Human SEMA5A (Semaphorin 5A) ELISA Kit
The Human (SEMA5A) Semaphorin 5A ELISA Kit measures Semaphorin 5A in samples. The plate has been pre-coated with Human SEMA5A antibody. SEMA5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA5A Antibody is added and binds to SEMA5A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA5A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA5A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1945Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.02 ng/mL
Detection Range
0.05-10 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Semaphorin 5A
Regulatory Status
For Research Use Only
