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Human SEMA5B (Semaphorin 5B) ELISA Kit

Human SEMA5B (Semaphorin 5B) ELISA Kit

The Human (SEMA5B) Semaphorin 5B ELISA Kit measures Semaphorin 5B in samples. The plate has been pre-coated with Human SEMA5B antibody. SEMA5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA5B Antibody is added and binds to SEMA5B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA5B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA5B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2275Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 10.25 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Neuroscience
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Semaphorin 5B
Regulatory Status For Research Use Only
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