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Human Serine Protease Inhibitor Kazal-Type 4 (SPINK4) ELISA Kit

Human Serine Protease Inhibitor Kazal-Type 4 (SPINK4) ELISA Kit

This ELISA kit is designed to detect Human Serine Protease Inhibitor Kazal-Type 4 (Human SPINK4). The assay plate has been pre-coated with mouse anti-Human SPINK4 monoclonal antibody. When the sample containing SPINK4 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human SPINK4 Antibody is added to the wells and binds to SPINK4 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human SPINK4 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE214
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 5.76 pg/mL
Detection Range 19.53-1250 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cell Biology
Background Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-6 homolog and SPINK4, is a secreted protein that contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged infertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Serine protease inhibitor Kazal-type 4
Regulatory Status For Research Use Only
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