The Human (SIgMAR1) SIgMa Non-opioid Intracellular Receptor 1 ELISA Kit measures Sigma non-opioid intracellular receptor 1 in samples. The plate has been pre-coated with Human SIGMAR1 antibody. SIGMAR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SIGMAR1 Antibody is added and binds to SIGMAR1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SIGMAR1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SIGMAR1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundFunctions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration (PubMed:16472803, PubMed:9341151).
Necessary for proper mitochondrial axonal transport in motor neurons, in particular the retrograde movement of mitochondria. Plays a role in protecting cells against oxidative stress-induced cell death via its interaction with RNF112 (By similarity). Source: UniProt Consortium (2025)
