• Human (SMPDL3B) Acid Sphingomyelinase-like Phosphodiesterase 3B ELISA Kit
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Human SMPDL3B (Acid Sphingomyelinase-like Phosphodiesterase 3B) ELISA Kit

Human SMPDL3B (Acid Sphingomyelinase-like Phosphodiesterase 3B) ELISA Kit

The Human (SMPDL3B) Acid Sphingomyelinase-like Phosphodiesterase 3B ELISA Kit measures Acid Sphingomyelinase-Like Phosphodiesterase 3B in samples. The plate has been pre-coated with Human SMPDL3B antibody. SMPDL3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SMPDL3B Antibody is added and binds to SMPDL3B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SMPDL3B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SMPDL3B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4893Hu
$595.00 USD
$458.00 USD $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.14 ng/mL
Detection Range 0.3-90 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Lipid-modulating phosphodiesterase (PubMed: 26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed: 26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Acid Sphingomyelinase-Like Phosphodiesterase 3B
Regulatory Status For Research Use Only
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