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Human SOCS-3 (Suppressors of Cytokine Signaling 3) ELISA Kit
Human SOCS-3 (Suppressors of Cytokine Signaling 3) ELISA Kit
The Human (SOCS-3) Suppressors of Cytokine Signaling 3 ELISA Kit measures Suppressors of Cytokine Signaling 3 in samples. The plate has been pre-coated with Human SOCS3 antibody. SOCS3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SOCS3 Antibody is added and binds to SOCS3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SOCS3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SOCS3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0657Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.02 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity and regulates IL6 signaling. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed: 15601820). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Suppressors of Cytokine Signaling 3
Regulatory Status
For Research Use Only
