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Human SPSB2 (Spry Domain-containing Socs Box Protein 2) ELISA Kit
Human SPSB2 (Spry Domain-containing Socs Box Protein 2) ELISA Kit
The Human (SPSB2) Spry Domain-containing Socs Box Protein 2 ELISA Kit measures SPRY domain-containing SOCS box protein 2 in samples. The plate has been pre-coated with Human SPSB2 antibody. SPSB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPSB2 Antibody is added and binds to SPSB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPSB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SPSB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3179Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.12 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology
Background
Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820, PubMed:21199876).
Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed:21199876).
Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed:21199876). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
SPRY domain-containing SOCS box protein 2
Regulatory Status
For Research Use Only
