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Human SRD5A1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1) ELISA Kit

Human SRD5A1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1) ELISA Kit

The Human (SRD5A1) 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit measures 3-oxo-5-alpha-steroid 4-dehydrogenase 1 in samples. The plate has been pre-coated with Human SRD5A1 antibody. SRD5A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SRD5A1 Antibody is added and binds to SRD5A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SRD5A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SRD5A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4880Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.029 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte 3-oxo-5-alpha-steroid 4-dehydrogenase 1
Regulatory Status For Research Use Only
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