1 1

Human Superoxide Dismutase [Cu-Zn] (SOD1) ELISA Kit

Human Superoxide Dismutase [Cu-Zn] (SOD1) ELISA Kit

This ELISA kit is designed to detect Human Superoxide Dismutase [Cu-Zn] (Human SOD1). The assay plate has been pre-coated with mouse anti-Human SOD1 monoclonal antibody. When the sample containing SOD1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human SOD1 Antibody is added to the wells and binds to SOD1 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human SOD1 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE219
$624.00 USD
$480.00 USD $624.00 USD
Sale Sold out
Packaging
2 weeks
Download PDF

Product Details

Species Reactivity Human
Sensitivity 29.48 pg/mL
Detection Range 62.5-4000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Cancer, Cell Biology, Metabolism, Neuroscience, Signal Transduction
Background SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Superoxide dismutase [Cu-Zn]
Regulatory Status For Research Use Only
View full details

Inquire about this product