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Human TJP1 (Tight Junction Protein Zo-1) ELISA Kit
Human TJP1 (Tight Junction Protein Zo-1) ELISA Kit
The Human (TJP1) Tight Junction Protein Zo-1 ELISA Kit measures Tight Junction Protein Zo-1 in samples. The plate has been pre-coated with Human TJP1 antibody. TJP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TJP1 Antibody is added and binds to TJP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TJP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TJP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4353hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.46 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Tags and Cell Markers
Background
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed: 7798316, PubMed: 9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed: 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed: 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Tight Junction Protein Zo-1
Regulatory Status
For Research Use Only
