The Human (TNFRSF14) Tumor Necrosis Factor Receptor Superfamily Member 14 ELISA Kit measures Tumor Necrosis Factor Receptor Superfamily Member 14 in samples. The plate has been pre-coated with Human TNFRSF14 antibody. TNFRSF14 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFRSF14 Antibody is added and binds to TNFRSF14 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNFRSF14 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TNFRSF14. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundReceptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed: 9462508, PubMed: 10754304, PubMed: 18193050, PubMed: 23761635). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed: 19915044, PubMed: 9153189, PubMed: 9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed: 10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed: 23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed: 18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (PubMed: 19915044) (By similarity). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed: 19915044) (By similarity). (Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1. (Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteTumor Necrosis Factor Receptor Superfamily Member 14
