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Human TOP2A (DNA Topoisomerase 2-alpha) ELISA Kit
Human TOP2A (DNA Topoisomerase 2-alpha) ELISA Kit
The Human (TOP2A) DNA Topoisomerase 2-alpha ELISA Kit measures DNA topoisomerase 2-alpha in samples. The plate has been pre-coated with Human TOP2A antibody. TOP2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TOP2A Antibody is added and binds to TOP2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TOP2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TOP2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6652Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.78 ng/L
Detection Range
20-3800 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Cancer
Background
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612).
May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
DNA topoisomerase 2-alpha
Regulatory Status
For Research Use Only
