Human TPAB (Treponema pallidum antibody) ELISA Kit
Human TPAB (Treponema pallidum antibody) ELISA Kit
The Human TPAB (Treponema pallidum antibody) ELISA Kit measures TP-Ab in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with TP-Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TP-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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