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Human TPI1 (Triosephosphate Isomerase) ELISA Kit

Human TPI1 (Triosephosphate Isomerase) ELISA Kit

The Human (TPI1) Triosephosphate Isomerase ELISA Kit measures Triosephosphate Isomerase in samples. The plate has been pre-coated with Human TPI1 antibody. TPI1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TPI1 Antibody is added and binds to TPI1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TPI1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TPI1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E6319Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 9.15 ng/L
Detection Range 20-3500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Metabolism
Background Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Triosephosphate Isomerase
Regulatory Status For Research Use Only
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