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Human TPMT (Thiopurine S-methyltransferase) ELISA Kit
Human TPMT (Thiopurine S-methyltransferase) ELISA Kit
The Human (TPMT) Thiopurine S-methyltransferase ELISA Kit measures Thiopurine S-methyltransferase in samples. The plate has been pre-coated with Human TPMT antibody. TPMT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TPMT Antibody is added and binds to TPMT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TPMT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TPMT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6266Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.29 mIU/mL
Detection Range
0.5-200 mIU/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Metabolism
Background
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed:657528, PubMed:18484748).
TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Thiopurine S-methyltransferase
Regulatory Status
For Research Use Only
