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Human TRIB3 (Tribbles Homolog 3) ELISA Kit
Human TRIB3 (Tribbles Homolog 3) ELISA Kit
The Human (TRIB3) Tribbles Homolog 3 ELISA Kit measures Tribbles Homolog 3 in samples. The plate has been pre-coated with Human TRIB3 antibody. TRIB3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TRIB3 Antibody is added and binds to TRIB3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIB3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TRIB3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4618Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.86 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed: 15781252, PubMed: 15775988). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed: 15781252, PubMed: 15775988). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed: 15781252, PubMed: 15775988). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed: 12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed: 15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed: 22977230). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Tribbles Homolog 3
Regulatory Status
For Research Use Only
