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Human Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) ELISA Kit
Human Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) ELISA Kit
This ELISA kit is designed to detect Human Triggering Receptor Expressed on Myeloid Cells 2 (Human TREM2). The assay plate has been pre-coated with mouse anti-Human TREM-2 monoclonal antibody. When the sample containing TREM-2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human TREM-2 Antibody is added to the wells and binds to TREM-2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human TREM-2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE177
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
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per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
16.98 pg/mL
Detection Range
54.69-3500 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Immunology, Neuroscience
Background
Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Triggering receptor expressed on myeloid cells 2
Regulatory Status
For Research Use Only
