1
/
of
1
Human TRIM63 (E3 Ubiquitin-protein Ligase Trim63) ELISA Kit
Human TRIM63 (E3 Ubiquitin-protein Ligase Trim63) ELISA Kit
The Human (TRIM63) E3 Ubiquitin-protein Ligase Trim63 ELISA Kit measures E3 ubiquitin-protein ligase TRIM63 in samples. The plate has been pre-coated with Human TRIM63 antibody. TRIM63 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TRIM63 Antibody is added and binds to TRIM63 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIM63 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TRIM63. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5218Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
3.13 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology, Immunology
Background
E3 ubiquitin-protein ligase TRIM63, also known as "MuRF1", is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
E3 ubiquitin-protein ligase TRIM63
Regulatory Status
For Research Use Only
