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Human TRPV4 (Transient Receptor Potential Cation Channel Subfamily V Member 4) ELISA Kit
Human TRPV4 (Transient Receptor Potential Cation Channel Subfamily V Member 4) ELISA Kit
The Human (TRPV4) Transient Receptor Potential Cation Channel Subfamily V Member 4 ELISA Kit measures Transient receptor potential cation Channel subfamily V member 4 in samples. The plate has been pre-coated with Human TrpV4 antibody. TrpV4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TrpV4 Antibody is added and binds to TrpV4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TrpV4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TrpV4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6305Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.99 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:18826956, PubMed:18695040, PubMed:29899501).
Also activated by heat, low pH, citrate and phorbol esters (PubMed:16293632, PubMed:18826956, PubMed:18695040, PubMed:25256292, PubMed:20037586, PubMed:21964574).
Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (PubMed:12724311, PubMed:18826956).
Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity).
Acts as a regulator of intracellular Ca2+ in synoviocytes (PubMed:19759329).
Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (PubMed:19759329).
Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040).
Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity).
Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity).
Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity).
Required for normal development and maintenance of bone and cartilage (PubMed:26249260).
In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus (PubMed:29899501).
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
(Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X.
(Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X.
(Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Transient receptor potential cation Channel subfamily V member 4
Regulatory Status
For Research Use Only
