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Human VCL (Vinculin) ELISA Kit

Human VCL (Vinculin) ELISA Kit

The Human (VCL) Vinculin ELISA Kit measures vinculin in samples. The plate has been pre-coated with Human VCL antibody. VCL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VCL Antibody is added and binds to VCL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VCL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VCL . The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0684Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 9.32 ng/L
Detection Range 20-3800 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction, Cardiovascular
Background Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte vinculin
Regulatory Status For Research Use Only
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